Automated Clinical Rapid Whole Exome Sequencing with the Covaris R230 Focused-ultrasonicator
DNA fragmentation is a critical step in the preparation of good quality libraries for next-generation sequencing (NGS). Covaris Adaptive Focused Acoustics® (AFA®) technology remains the gold standard in reproducible and robust fragmentation, especially relevant while working with precious clinical samples. Automated workflows are needed more than ever to meet the increasing demand in sample throughput and sequencing capacity, whilst minimizing the risk of human errors. The newest line of Covaris AFA-TUBE consumables allows easy automation integration and the R230 Focused-ultrasonicator can integrate with any liquid handler on-deck or deck-adjacent. Here we present a case study with Radboudumc using our next generation of plate-based Focused-ultrasonicator, the R230, and a streamlined approach to high-throughput NGS library preparation.